Easily encapsulate single cells inside highly-monodisperse W/O/W double emulsions at a very high rate
Fluorescence-activated cell sorting is a powerful tool for selecting a specific cellular or bacterial cell type from a complex population. However, it often requires the use of chemicals which can damage the cells, and the selection of single bacteria can be difficult to complete due to low fluorescence signals. Compartmentalizing single bacteria inside double emulsions using microfluidics allows for bacterial growth in the droplets, confinement of the fluorescence signal inside the droplet, and the specific analysis of the bacteria’s phenotype via isolation. Overall, it allows for the FACS analysis of each specific phenotype with measurable signals and without cell damage.
Learn how to use our double emulsion platform to encapsulate bacteria in W/O/W DE
1. Introduction to FACS sorting: technology, advantages, and drawbacks (5 min)
2. Presentation of the platform: encapsulation of single bacteria in DE (15 min)
3. Microscopy characterization and FACS sorting (15 min)
4. Interactive Q&A session to discuss your application needs with our experts (25 min)
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Current solutions for organ-on-a-chip experiments can be cumbersome and are mostly designed for microfluidic experts. Fluigent’s new Omi device meets the need for a simple, easy-to-use device in organ-on-a-chip studies..